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Inorganic Cations
Standards
Inorganic Cations
in Beaverages
Inorganic Cations
Std. 10um capillary
Inorganic Anions
Std./urine
Amino Acids in Beer
Protease and Reverse
Transcriptase Inhibitors
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In capillary electrophoresis, small charged analytes with a high equivalent
conductivity can be detected in the ppb and sub-ppb range as long as low
conducting background electrolytes are used. In order to separate inorganic cations,
buffers consisting of a mixture of morpholinoethanesulfonic acid (MES) and Histidin
(His) at a slightly acidic pH are suitable. For anionic analytes, basic background
electrolytes, such as a mixture of arginine (Arg) and sorbic acid give best results.
Contactless conductivity detection is not limited to inorganic ions. Organic ions
usually exhibit a lower inherent conductivity compared with inorganic ions, however,
they can be detected using indirect conductivity detection. In order to limit the
baseline noise of background electrolytes with a higher conductivity, capillaries with
small I.D.s, e.g. 25 µm, are used.